98 .
RHIZOBIUM MEDIUM
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Selected strain: Shinella kummerowiae DSM 19334
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Equipment needed:
Autoclave
Centrifuge
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 1 | g | ||
|
|
Mannitol | 10 | g | ||
|
|
Agar | 15 | g | ||
|
|
Soil extract | 200 | ml | ||
|
|
Distilled water | 800 | ml | ||
| 1 Adjust pH to 7.0. | |||||
Soil extract #
200
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
air-dried garden soil | 80.0 | g | ||
|
|
Na2CO3 | 0.2 | g | ||
|
|
Distilled water | 200.0 | ml | ||
| 1 Autoclave soil suspension for one hour at 121°C. To obtain a clear supernatant allow to settle and centrifuge. Adjust pH to 7.2. | |||||