98
98 .
RHIZOBIUM MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Shinella kummerowiae DSM 19334
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Main sol. 98 #
ml
Equipment needed: Centrifuge
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 1 | g | 1 | - | |
| Mannitol | 10 | g | 10 | 54.894 | |
| Agar | 15 | g | 15 | 44.599 | |
| Soil extract | 200 | ml | - | - | |
| Distilled water | 800 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| air-dried garden soil | 80.0 | g | 400 | - | |
| Na2CO3 | 0.2 | g | 1 | 9.435 | |
| Distilled water | 200.0 | ml | - | - | |
| 1 Autoclave soil suspension for one hour at 121°C. To obtain a clear supernatant allow to settle and centrifuge. Adjust pH to 7.2. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Centrifuge |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 151 |
| Strains with modifications: | 3 |
Shinella kummerowiae DSM 19334
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| air-dried garden soil | 80 | - |
| Agar | 15 | 44.599 |
| Mannitol | 10 | 54.894 |
| Yeast extract | 1 | - |
| Na2CO3 | 0.2 | 1.887 |
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Cultivation metadata from 151 strains
| pH | 6 - 8 | 16 strains |
| pH | 4 - 6 | 12 strains |
| pH | 8 - 10 | 10 strains |
| pH | 10 - 12 | 5 strains |
| phyla | 1 | 160 strains |
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