986
986 .
PARAMALEDIVIBACTER MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 986 #
ml
Equipment needed: Autoclave
Filter
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(SIGMA) |
30.00 | g | 29.97 | - | |
| NH4Cl | 1.00 | g | 0.999 | 18.676 | |
| K2HPO4 | 0.30 | g | 0.3 | 1.721 | |
| KH2PO4 | 0.30 | g | 0.3 | 2.202 | |
| Peptone | 0.50 | g | 0.5 | - | |
| Yeast extract | 0.50 | g | 0.5 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| NaHCO3 | 2.00 | g | 1.998 | 23.784 | |
| D-Glucose | 4.00 | g | 3.996 | 22.18 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.25 | g | 0.25 | 1.422 | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.04 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate, glucose, vitamins, cysteine and sulfide), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins, glucose, sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins should be sterilized by filtration. The pH of the complete medium should be 7.0. | |||||
Solution occurs in 305
other media (first defined in Medium 120).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 20 | mg | 0.02 | 0.082 |
| Folic acid | 20 | mg | 0.02 | 0.045 |
| Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
| Thiamine HCl | 50 | mg | 0.05 | 0.148 |
| Riboflavin | 50 | mg | 0.05 | 0.133 |
| Nicotinic acid | 50 | mg | 0.05 | 0.406 |
| Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
| Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
| p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
| (DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 11.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sea Salt | 29.97 | - |
| D-Glucose | 3.996 | 22.18 |
| NaHCO3 | 1.998 | 23.784 |
| NH4Cl | 0.999 | 18.676 |
| Peptone | 0.5 | - |
| Show more | ||
