971 .
HALOMONAS MAGADIENSIS MEDIUM
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* Medium for Salsuginibacillus kocurii DSM 18087
Modify: add 80g/l NaCl and 20.0 g/l Na2CO3; pH: 11
Growth conditions: 30 °C
Equipment needed:
Autoclave
Final pH:
* 11.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Glucose | 10.0 | g | ||
|
|
Peptone
(Difco) |
5.0 | g | ||
|
|
Yeast extract
(Difco) |
5.0 | g | ||
|
|
KH2PO4 | 1.0 | g | ||
|
|
MgSO4 x 7 H2O | 0.2 | g | ||
|
|
NaCl | 40.0 | g | ||
|
|
Na2CO3 | 10.0 | g | ||
|
|
Agar | 20.0 | g | ||
|
|
NaCl | 80.0 | g/l | ||
|
|
Na2CO3 | 20.0 | g/l | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 NaCl and Na2CO3 were autoclaved separately and added to the organic components at 60°C before pouring the agar media. Final pH should be about 10.0. | |||||