Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
This strain uses the standard medium without modifications.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.14 | g | 0.14 | 1.027 | |
MgCl2 x 6 H2O | 0.20 | g | 0.2 | 0.982 | |
CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.018 | |
NH4Cl | 0.54 | g | 0.539 | 10.075 | |
Trace element solution | 1.00 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
Na2CO3 | 1.50 | g | 1.497 | 14.124 | |
Na-pyruvate | 2.20 | g | 2.196 | 19.952 | |
Yeast extract
(OXOID) |
0.10 | g | 0.1 | - | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.30 | g | 0.299 | 1.705 | |
Na2S x 9 H2O | 0.30 | g | 0.299 | 1.247 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except carbonate, pyruvate, yeast extract, vitamins, and reducing agents) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add pyruvate, yeast extract, vitamins, cysteine, and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Stock solutions of vitamins and pyruvate should be sterilized by filtration. The pH of the complete medium should be 7.0. | |||||
2 Note: Use 10% (v/v) as inoculum. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid
(NTA) |
12.80 | g | 12.8 | 66.967 | |
FeCl2 x 4 H2O | 1.00 | g | 1 | 5.03 | |
MnCl2 x 4 H2O | 0.10 | g | 0.1 | 0.505 | |
CoCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnCl2 | 0.10 | g | 0.1 | 0.734 | |
CuCl2 | 0.02 | g | 0.02 | 0.149 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
NiCl2 x 6 H2O | 0.10 | g | 0.1 | 0.772 | |
NaCl | 1.00 | g | 1 | 17.112 | |
Na2SeO3 x 5 H2O | 0.03 | g | 0.03 | 0.114 | |
Na2WO4 x 2 H2O | 0.04 | g | 0.04 | 0.121 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 20 | mg | 0.02 | 0.082 |
Folic acid | 20 | mg | 0.02 | 0.045 |
Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
Thiamine HCl | 50 | mg | 0.05 | 0.148 |
Riboflavin | 50 | mg | 0.05 | 0.133 |
Nicotinic acid | 50 | mg | 0.05 | 0.406 |
Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
(DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
Distilled water | 1000 | ml | - | - |
Last modified: | 27.10.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 7.0 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 19 |
Strains with modifications: | 18 |
No modifications necessary for this strain.
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Na-pyruvate | 2.196 | 19.952 |
Na2CO3 | 1.497 | 14.124 |
NH4Cl | 0.539 | 10.075 |
L-Cysteine HCl x H2O | 0.299 | 1.705 |
Na2S x 9 H2O | 0.299 | 1.247 |
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