92a
92a .
MODIFIED MEDIUM 92
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Luteococcus peritonei DSM 15826
* Modification: Aerobic-microaerophilic
Growth conditions: 30 °C
Main sol. 92a #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium 92 | 990 | ml | - | - | |
| Vitamin solution | 10 | ml | - | - | |
| 1 Prepare medium 92 and subsequently add vitamins. Adjust pH to 7.0 - 7.2 | |||||
Solution occurs in 3
other media (first defined in Medium 11a).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.00 | mg | 0.002 | 0.008 |
| Folic acid | 2.00 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.00 | mg | 0.01 | 0.049 |
| Thiamine-HCl x 2 H2O | 5.00 | mg | 0.005 | 0.013 |
| Riboflavin | 5.00 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.00 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.00 | mg | 0.005 | 0.01 |
| p-Aminobenzoic acid | 1.00 | mg | 1.0e-3 | 0.007 |
| Vitamin B12 | 0.01 | mg | 1.0e-5 | 7.4e-6 |
| Distilled water | 1000.00 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trypticase soy broth | 30 | g | 30 | - | |
| Yeast extract | 3 | g | 3 | - | |
| Agar , if necessary | 15 | g | 15 | 44.599 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.0 - 7.2. | |||||
Metadata
| Last modified: | 11.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Trypticase soy broth | 29.7 | - |
| Agar | 14.85 | 44.153 |
| Yeast extract | 2.97 | - |
| Pyridoxine hydrochloride | 1.0e-4 | 4.9e-4 |
| Nicotinic acid | 5.0e-5 | 4.1e-4 |
| Show more | ||
