This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 270.00 | g | 270 | 4620.12 | |
NaBr | 0.10 | g | 0.1 | 0.972 | |
MgSO4 x 7 H2O | 20.00 | g | 20 | 81.144 | |
KCl | 5.00 | g | 5 | 67.069 | |
NH4Cl | 2.00 | g | 2 | 37.39 | |
Tris-HCl | 12.00 | g | 12 | - | |
Yeast extract
(Difco) |
1.00 | g | 1 | - | |
Glucose | 2.00 | g | 2 | 11.101 | |
Distilled water | 1000.00 | ml | - | - | |
KH2PO4 solution 5%(w/v) | 2.50 | ml | - | - | |
CaCl2 solution 10%(w/v) | 0.50 | ml | - | - | |
FeCl2/MnCl2 solution | 0.25 | ml | - | - | |
1 Prepare the medium, and adjust the pH to 7.6 with 5M NaOH. | |||||
2 Autoclave and when the medium has cooled add 2.5 ml of the phosphate solution, 0.5 ml of the calcium chloride solution, and 0.25 ml of the Fe/Mn solution. This strain will not grow in normal media for halobacteria, such as DSM medium 372! |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
HCl
(25%) |
6.5 | ml | 1.625 | 44.569 |
FeCl2 x 7 H2O | 1.5 | g | 1.5 | 11.834 |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 |
H3BO3 | 62.0 | mg | 0.062 | 1.003 |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
CuCl2 x 2 H2O | 17.0 | mg | 0.017 | 0.1 |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 50 | g | 50 | 367.417 | |
Distilled water | 1000 | ml | - | - | |
1 Prepare and sterilise separately |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
CaCl2 x 6 H2O | 100 | g | 100 | 456.463 | |
Distilled water | 1000 | ml | - | - | |
1 Prepare and sterilise separately |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
FeCl2 x 4 H2O | 20 | g | 20 | 100.598 | |
MnCl2 x 4 H2O | 20 | g | 20 | 101.059 | |
Distilled water | 1000 | ml | - | - | |
1 Prepare and sterilise separately |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 7.6 |
Edit in Medium builder |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 270 | 4620.12 |
MgSO4 x 7 H2O | 20 | 81.144 |
Tris-HCl | 12 | - |
KCl | 5 | 67.069 |
NH4Cl | 2 | 37.39 |
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