MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 926 . ALKALIPHILIC THERMOCOCCUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Thermococcus acidaminovorans DSM 11906

* Modification: To the sterile, anaerobic, mineral medium add 0.08 ml polysulphide/10 ml medium, casamino acids to a final concentration of 0.2%. The may be precipitation of material and the medium will turn pale yellow due to the addition of the polysulphide. The colour will disappear as the strain grows.

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 12-24 h anaerobic 85 °C pH 7.50

Main sol. 926 #

ml
Equipment needed: Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 27.70 g 13.85 236.995
MgSO4 x 7 H2O 7.00 g 3.5 14.2
MgCl2 x 6 H2O 5.50 g 2.75 13.527
KCl 0.65 g 0.325 4.359
NaBr 0.10 g 0.05 0.486
NaHCO3 0.32 g 0.16 1.905
K2HPO4 1.00 g 0.5 2.871
CaCl2 x 2 H2O 0.05 mg 2.5e-5 1.7e-4
KI 15.00 mg 0.008 0.029
H3BO3 0.03 g 0.015 0.243
Modified Wolin's mineral solution II 20.00 ml - -
Casamino acids* 0.20 % - -
Distilled water 2000.00 ml - -
1 Prepare the medium anaerobically under nitrogen. Do not adjust the pH.
2 Prepare separate anaerobic stock solutions of, Casamino acids(10%), Yeast extract (10%), and glycine (2M = 150 g/l). A 0.5M polysulphide solution is prepared by dissolving 12.0 g Na2S x 9 H2O in oxygen free water, followed by adding 1.6 g sulphur - the solution will be dark yellow.

Modified Wolin's mineral solution II #

1000
Solution occurs in 24 other media (first defined in Medium 700).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
MgSO4 x 7 H2O 3.000 g 3 12.172
MnSO4 x H2O 0.500 g 0.5 2.958
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CoSO4 x 7 H2O 0.180 g 0.18 1.161
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnSO4 x 7 H2O 0.180 g 0.18 0.626
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.020 g 0.02 0.042
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Na2SeO3 x 5 H2O 0.300 mg 3.0e-4 0.001
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 15.02.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Anaerobic workbench
Edit in Medium builder

Associated strains

All strains for this medium: 2
Strains with modifications: 2
Thermococcus acidaminovorans DSM 11906

  0.2 % Casamino acids

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.75 13.527
K2HPO4 0.5 2.871
KCl 0.325 4.359
NaHCO3 0.16 1.905
NaBr 0.05 0.486
Show more
CSV
JSON

Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature hyperthermophilic 2 strains