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DSMZ

DSMZ 899 . CALDANAEROBACTER MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 899 #

ml
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 0.99 18.51
K2HPO4 0.30 g 0.297 1.705
KH2PO4 0.30 g 0.297 2.183
MgCl2 x 6 H2O 1.00 g 0.99 4.87
CaCl2 x 2 H2O 0.10 g 0.099 0.673
KCl 0.20 g 0.198 2.656
NaCl 0.60 g 0.594 10.165
Modified Wolin's mineral solution 10.00 ml - -
Yeast extract 2.00 g 1.98 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Na2CO3 1.00 g 0.99 9.342
D-Glucose 4.00 g 3.96 21.983
Na2S2O3 x 5 H2O 2.50 g 2.475 9.973
L-Cysteine HCl x H2O 0.50 g 0.495 2.819
Na2S x 9 H2O 0.45 g 0.446 1.855
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, glucose, thiosulfate, cysteine and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, thiosulfate (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The pH of the complete medium should be at 7.0.

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 07.11.22
Source: DSMZ
Synonyms: THERMOANAEROBACTER SUBTERRANEUS MEDIUM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 4

Molecular composition

Compound Conc. [g/L] Conc. [mM]
D-Glucose 3.96 21.983
Na2S2O3 x 5 H2O 2.475 9.973
Yeast extract 1.98 -
NH4Cl 0.99 18.51
Na2CO3 0.99 9.342
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
oxygen anaerobe 4 strains
temperature thermophilic 4 strains