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DSMZ

DSMZ 897 . IGNICOCCUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Ignicoccus hospitalis DSM 18386

* Modification: Prepare medium without meat extract.

Growth conditions: anaerobic 90 °C

Main sol. 897 #

ml
Equipment needed: Hungate tubes Gassing station Water bath
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 13.65 g 13.542 231.719
MgSO4 x 7 H2O 3.50 g 3.472 14.088
MgCl2 x 6 H2O 2.75 g 2.728 13.419
KH2PO4 0.50 g 0.496 3.645
(NH4)2SO4 0.25 g 0.248 1.877
CaCl2 x 2 H2O 0.38 g 0.377 2.564
KCl 0.33 g 0.327 4.391
NaBr 0.05 g 0.05 0.482
H3BO3 15.00 mg 0.015 0.241
SrCl2 x 6 H2O
(0.1% w/v) 		7.00 ml 0.007 0.026
KI
(0.01% w/v) 		0.50 ml 5.0e-5 1.9e-4
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Sulfur
(powdered) 		5.00 g 4.96 154.672
Meat extract* 1.00 g 0.992 -
Na2S x 9 H2O 0.20 g 0.198 0.826
Distilled water 1000.00 ml - -
1 Dissolve ingredients except sulfur, meat extract and sulfide, adjust pH to 5.0 - 5.5 with 2 N H2SO4 and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Distribute medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials already containing the appropriate amount of sulfur, only to 30% of their volume to allow for a large head space. For sterilization heat cultivation vessels in boiling water for 1 -2 hours on each of 3 successive days. Add meat extract and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere.
2 After inoculation pressurize the vessels with sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure.

Metadata

Last modified: 27.10.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 5.0 - 5.5
Equipment needed: Gassing station, Hungate tubes, Water bath
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Strains with modifications: 1
Ignicoccus hospitalis DSM 18386

  Meat extract

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
NaCl 13.542 231.719
Sulfur 4.96 154.672
MgSO4 x 7 H2O 3.472 14.088
MgCl2 x 6 H2O 2.728 13.419
KH2PO4 0.496 3.645
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Cultivation metadata from 3 strains BacDive

pH 4 - 6 3 strains
pH 6 - 8 3 strains
pH 2 - 4 1 strain
phyla 1 3 strains
nutrition chemolithoautotroph 3 strains
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