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DSMZ

DSMZ 891 . SME MEDIUM MODIFIED FOR IS7

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 891 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 30 513.347
MgSO4 x 7 H2O 0.70 g 0.7 2.84
MgCl2 x 6 H2O 0.55 g 0.55 2.705
KCl 0.65 g 0.65 8.719
NaBr 0.10 g 0.1 0.972
NH4Cl 0.15 g 0.15 2.804
K2HPO4 0.15 g 0.15 0.861
CaCl2 x 2 H2O 0.50 g 0.5 3.401
Modified Wolin's mineral solution II 10.00 ml - -
Sulfur
(powdered) 		0.50 g 0.5 15.591
Distilled water 1000.00 ml - -
1 Adjust pH to 7.0 using H2SO4.
2 Prepare the medium under H2 gas and fill 20 ml medium into 100 ml serum bottles and seal with a rubber stopper. Steam/heat medium in an almost boiling waterbath for 3 hours on each of 3 successive days to sterilize.
3 Add bicarbonate from a autoclaved, neutralized stock solution.
NaHCO3 2.00 g/l 2 23.808
4 Give an atmosphere of H2/CO2/O2 (78/20/2) with an overpressure of two atmospheres.

Modified Wolin's mineral solution II #

1000
Solution occurs in 23 other media (first defined in Medium 700).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
MgSO4 x 7 H2O 3.000 g 3 12.172
MnSO4 x H2O 0.500 g 0.5 2.958
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CoSO4 x 7 H2O 0.180 g 0.18 1.161
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnSO4 x 7 H2O 0.180 g 0.18 0.626
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.020 g 0.02 0.042
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Na2SeO3 x 5 H2O 0.300 mg 3.0e-4 0.001
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 08.05.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
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Gas composition

  • 78 % H2
  • 20 % CO2
  • 2 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2 23.808
KCl 0.65 8.719
MgCl2 x 6 H2O 0.55 2.705
Sulfur 0.5 15.591
NH4Cl 0.15 2.804
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain