864
864 .
HALOTHIOBACILLUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 864 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 29.00 | g | 28.856 | 493.767 | |
| (NH4)2SO4 | 1.00 | g | 0.995 | 7.53 | |
| MgSO4 x 7 H2O | 1.50 | g | 1.493 | 6.056 | |
| CaCl2 x 2 H2O | 0.42 | g | 0.418 | 2.843 | |
| KCl | 0.70 | g | 0.697 | 9.343 | |
| Trace element solution SL-11 | 1.00 | ml | - | - | |
|
Bromothymol blue
(0.1% w/v) |
4.00 | ml | 0.004 | 0.006 | |
| K2HPO4 | 0.50 | g | 0.498 | 2.856 | |
| Na2S2O3 x 5 H2O | 5.00 | g | 4.975 | 20.046 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except hydrogenphosphate and thiosulfate, adjust pH to 6.5, then dispense medium into culture vessels and autoclave. Add hydrogenphosphate and thiosulfate from sterile stock solutions. Adjust pH of complete medium to 6.5 - 7.0. | |||||
Solution occurs in 22
other media (first defined in Medium 722).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA x 2 H2O | 5.2 | g | 5.2 | 15.467 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml. | |||||
Metadata
| Last modified: | 07.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 6.5 - 7.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 28.856 | 493.767 |
| Na2S2O3 x 5 H2O | 4.975 | 20.046 |
| MgSO4 x 7 H2O | 1.493 | 6.056 |
| (NH4)2SO4 | 0.995 | 7.53 |
| KCl | 0.697 | 9.343 |
| Show more | ||
