852 .
GYP GLUCOSE-YEAST-PEPTONE MEDIUM
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Selected strain: Sporolactobacillus nakayamae subsp. racemicus DSM 11698
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Equipment needed:
Filter
Final pH:
6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Glucose | 20 | g | ||
|
|
Yeast extract | 10 | g | ||
|
|
Peptone
(tryptic) |
10 | g | ||
|
|
Na-acetate | 10 | g | ||
|
|
Salt solution | 5 | ml | ||
|
|
Distilled water | 1000 | ml | ||
|
|
Agar , for solid medium | 10 | g | ||
| 1 For solid media add 10 g/l bacteriological agar. Adjust to pH 6.8. After sterilization of the medium add filter sterilized salt solution. | |||||
Salt solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
MgSO4 x 7 H2O | 40 | g |
|
|
MnSO4 x 4 H2O | 2 | g |
|
|
FeSO4 x 7 H2O | 2 | g |
|
|
NaCl | 2 | g |
|
|
Distilled water | 1000 | ml |