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DSMZ

DSMZ 851 . GLUCOSE SULFIDE MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Tetrasphaera australiensis DSM 13193

This strain uses the standard medium without modifications.

Growth conditions: 28 °C

Main sol. 851 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 0.15 g 0.15 0.833
Yeast extract 1.00 g 1 -
(NH4)2SO4 0.50 g 0.5 3.784
CaCO3 0.10 g 0.1 0.999
Ca(NO3)2 0.10 g 0.1 0.609
KCl 0.05 g 0.05 0.671
K2HPO4 0.05 g 0.05 0.287
MgSO4 x 7 H2O 0.05 g 0.05 0.203
Na2S x 9 H2O 0.20 g 0.2 0.833
Vitamin solution 10.00 ml - -
Distilled water 990.00 ml - -
1 Adjust pH to 7.3.

Vitamin solution #

1000
Solution occurs in 3 other media (first defined in Medium 11a).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.00 mg 0.002 0.008
Folic acid 2.00 mg 0.002 0.005
Pyridoxine hydrochloride 10.00 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.00 mg 0.005 0.013
Riboflavin 5.00 mg 0.005 0.013
Nicotinic acid 5.00 mg 0.005 0.041
Calcium pantothenate 5.00 mg 0.005 0.01
p-Aminobenzoic acid 1.00 mg 1.0e-3 0.007
Vitamin B12 0.01 mg 1.0e-5 7.4e-6
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 3
Tetrasphaera australiensis DSM 13193

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 1 -
(NH4)2SO4 0.5 3.784
Na2S x 9 H2O 0.2 0.833
Glucose 0.15 0.833
CaCO3 0.1 0.999
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen obligate aerobe 1 strain
temperature mesophilic 3 strains