84
84 .
ROLLED OATS MINERAL MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Rugosimonospora acidiphila DSM 45227
* Modification: Adjust pH of final medium to 5.5 - 6.0.
Growth conditions: aerobic
28 °C
Main sol. 84 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Rolled oats | 20 | g | 20 | - | |
| Agar | 20 | g | 20 | - | |
| Trace element solution | 1 | ml | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 pH 7.2 | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| FeSO4 x 7 H2O | 0.1 | g | 1 | 3.597 |
| MnCl2 x 4 H2O | 0.1 | g | 1 | 5.053 |
| ZnSO4 x 7 H2O | 0.1 | g | 1 | 3.478 |
| Distilled water | 100.0 | ml | - | - |
Metadata
| Last modified: | 08.04.24 |
| Source: | DSMZ |
| Synonyms: | ISP 3 Medium |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | * 5.5 - 6.0 |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 20 | - |
| Rolled oats | 20 | - |
| MnCl2 x 4 H2O | 1.0e-3 | 0.005 |
| FeSO4 x 7 H2O | 1.0e-3 | 0.004 |
| ZnSO4 x 7 H2O | 1.0e-3 | 0.003 |
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Cultivation metadata from 1523 strains
| halophily | non-halophilic | 2 strains |
| halophily | moderately halophilic | 1 strain |
| pH | 6 - 8 | 94 strains |
| pH | 4 - 6 | 76 strains |
| pH | 8 - 10 | 53 strains |
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