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DSMZ

DSMZ 841 . MSV-THIOTRIX-MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 841 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution I 991.0 ml - -
Solution II 8.0 ml - -
Solution III 1.0 ml - -
Solution IV 0.1 ml - -
Agar 15.0 g/l - -
1 Final pH of appr. 7.4 should be controlled sterile.

Solution I #

991
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.500 g 0.505 3.818
MgSO4 x 7 H2O 0.100 g 0.101 0.409
CaCl2 x 2 H2O 0.050 g 0.05 0.343
K2HPO4 0.110 g 0.111 0.637
KH2PO4 0.085 g 0.086 0.63
FeCl3 x 6 H2O 2.000 mg 0.002 0.007
Na2-EDTA 3.000 mg 0.003 0.009
Na-acetate 0.546 g 0.551 6.716
Na2S2O3 x 5 H2O 0.400 g 0.404 1.626
Distilled water 991.000 ml - -
1 Autoclave for 20 min. at 121°C.

Solution II #

50
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 2.625 g 52.5 624.948
Distilled water 50.000 ml - -
1 NaHCO3 2.625 g per 50 ml distilled water
2 Autoclave for 20 min. at 121°C and add 8 ml/l sterile solution II shortly, before inoculation of bacterial culture.

Solution III #

1
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Standard vitamin solution 1 ml - -
1 H3P-vitamine solution (see medium 428)
2 Filter sterilize vitamine solution and add 1 ml/l medium.

Solution IV #

10
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Inositol 1 g 100 555.062
Distilled water 10 ml - -
1 Final pH of appr. 7.4 should be controlled sterile.
2 10% inositol (1g/10 ml distilled water)
3 Filter sterilize solution IV and add 0.1 ml/l medium.

Standard vitamin solution #

100
Solution occurs in 3 other media (first defined in Medium 428).
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Riboflavin 10.0 mg 0.1 0.266
Thiamine-HCl x 2 H2O 50.0 mg 0.5 1.339
Nicotinic acid 50.0 mg 0.5 4.061
Pyridoxine hydrochloride 50.0 mg 0.5 2.431
Calcium pantothenate 50.0 mg 0.5 1.049
Biotin 0.1 mg 1.0e-3 0.004
Folic acid 0.2 mg 0.002 0.005
Vitamin B12 1.0 mg 0.01 0.007
Distilled water 100.0 ml - -
1 Filter sterilize (0.2 µm pore-size) and store at 4°C.

Metadata

Last modified: 16.01.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.4
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na-acetate 0.546 6.656
(NH4)2SO4 0.5 3.784
NaHCO3 0.42 5
Na2S2O3 x 5 H2O 0.4 1.612
K2HPO4 0.11 0.632
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain