830c
830c .
REACTIVATION WITH LIQUID MEDIUM 830
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Novosphingobium lentum DSM 13663
* Modification: With or without 15 mg/l pentachlorophenol
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 20 °C
Main sol. 830c #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| 830. R2A MEDIUM | 1000 | ml | - | - | |
| Pentachlorophenol* | 15 | mg/l | - | - | |
| 1 Rehydrate and grow lyophilized cells from ampoules in liquid broth 830. Subsequent subculturing may be carried out in liquid medium or on agar plates. | |||||
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.50 | g | 0.5 | - | |
|
Proteose peptone
(Difco no. 3) |
0.50 | g | 0.5 | - | |
| Casamino acids | 0.50 | g | 0.5 | - | |
| Glucose | 0.50 | g | 0.5 | 2.775 | |
|
Starch
(soluble) |
0.50 | g | 0.5 | - | |
| Na-pyruvate | 0.30 | g | 0.3 | 2.726 | |
| K2HPO4 | 0.30 | g | 0.3 | 1.722 | |
| MgSO4 x 7 H2O | 0.05 | g | 0.05 | 0.203 | |
| Agar | 15.00 | g | 15 | 44.599 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Glucose | 0.5 | 2.775 |
| Yeast extract | 0.5 | - |
| Proteose peptone | 0.5 | - |
| Casamino acids | 0.5 | - |
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Cultivation metadata from 94 strains
| halophily | non-halophilic | 1 strain |
| pH | 6 - 8 | 33 strains |
| pH | 8 - 10 | 16 strains |
| pH | 4 - 6 | 11 strains |
| pH | 10 - 12 | 2 strains |
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