830c
830c .
REACTIVATION WITH LIQUID MEDIUM 830
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Delftia acidovorans DSM 17854
* Modification: Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 830. Subsequent subculturing may be carried out in liquid medium or on agar plates or in medium 465 plus taurine
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 30 °C
Main sol. 830c #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| 830. R2A MEDIUM | 1000 | ml | - | - | |
| 1 Rehydrate and grow lyophilized cells from ampoules in liquid broth 830. Subsequent subculturing may be carried out in liquid medium or on agar plates. | |||||
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.50 | g | 0.5 | - | |
|
Proteose peptone
(Difco no. 3) |
0.50 | g | 0.5 | - | |
| Casamino acids | 0.50 | g | 0.5 | - | |
| Glucose | 0.50 | g | 0.5 | 2.775 | |
|
Starch
(soluble) |
0.50 | g | 0.5 | - | |
| Na-pyruvate | 0.30 | g | 0.3 | 2.726 | |
| K2HPO4 | 0.30 | g | 0.3 | 1.722 | |
| MgSO4 x 7 H2O | 0.05 | g | 0.05 | 0.203 | |
| Agar | 15.00 | g | 15 | 44.599 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Glucose | 0.5 | 2.775 |
| Yeast extract | 0.5 | - |
| Proteose peptone | 0.5 | - |
| Casamino acids | 0.5 | - |
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Cultivation metadata from 94 strains
| halophily | non-halophilic | 1 strain |
| pH | 6 - 8 | 33 strains |
| pH | 8 - 10 | 16 strains |
| pH | 4 - 6 | 11 strains |
| pH | 10 - 12 | 2 strains |
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