830
830 .
R2A MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Thauera aminoaromatica DSM 25461
* Modification: With 5 ml vitamin solution, see e.g. medium 461
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 3-7 days
aerobic
28 °C
Main sol. 830 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(BD 212750) |
0.50 | g | - | - | |
|
Proteose peptone
(Difco no. 3) |
0.50 | g | - | - | |
| Casamino acids | 0.50 | g | - | - | |
| Glucose | 0.50 | g | - | - | |
|
Starch
(soluble) |
0.50 | g | - | - | |
| Na-pyruvate | 0.30 | g | - | - | |
| K2HPO4 | 0.30 | g | - | - | |
| MgSO4 x 7 H2O | 0.05 | g | - | - | |
| Agar | 15.00 | g | - | - | |
| Vitamin solution* | 5.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C. | |||||
| 2 Note: this equals BD 218263 | |||||
Solution occurs in 15
other media (first defined in Medium 461).
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Vitamin B12 | 50 | mg | 0.05 | 0.037 | |
| Pantothenic acid | 50 | mg | 0.05 | 0.228 | |
| Riboflavin | 50 | mg | 0.05 | 0.133 | |
| Pyridoxamine hydrochloride | 10 | mg | 0.01 | 0.049 | |
| Biotin | 20 | mg | 0.02 | 0.082 | |
| Folic acid | 20 | mg | 0.02 | 0.045 | |
| Nicotinic acid | 25 | mg | 0.025 | 0.203 | |
| Nicotine amide | 25 | mg | 0.025 | 0.205 | |
| alpha-lipoic acid | 50 | mg | 0.05 | 0.242 | |
| p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 | |
| Thiamine-HCl x 2 H2O | 50 | mg | 0.05 | 0.134 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Stir for some hours, filter sterilize the solution. | |||||
Metadata
| Last modified: | 23.05.25 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Glucose | 0.5 | 2.775 |
| Casamino acids | 0.5 | - |
| Starch | 0.5 | - |
| Proteose peptone | 0.5 | - |
| Show more | ||
Cultivation metadata from 2002 strains
| halophily | non-halophilic | 17 strains |
| halophily | moderately halophilic | 2 strains |
| pH | 6 - 8 | 499 strains |
| pH | 4 - 6 | 264 strains |
| pH | 8 - 10 | 249 strains |
| Show more | ||