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DSMZ

DSMZ 830 . R2A MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Nocardioides ginsengagri DSM 21362

* Modification: 1 drop of 10x Wolin Vitamine Solution (see medium 141) and 10 microliter trace element solution SL-10 (see medium 320) for better growth in liquid

Growth conditions: aerobic 28 °C pH 7.20

Main sol. 830 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(BD 212750) 		0.50 g - -
Proteose peptone
(Difco no. 3) 		0.50 g - -
Casamino acids 0.50 g - -
Glucose 0.50 g - -
Starch
(soluble) 		0.50 g - -
Na-pyruvate 0.30 g - -
K2HPO4 0.30 g - -
MgSO4 x 7 H2O 0.05 g - -
Agar 15.00 g - -
Modified Wolin's mineral solution* 1.00 ml - -
Trace element solution SL-10* 0.01 ml - -
Distilled water 1000.00 ml - -
1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.
2 Note: this equals BD 218263

Modified Wolin's mineral solution * #

1000
Solution occurs in 168 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Trace element solution SL-10 * #

1000
Solution occurs in 267 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 23.05.25
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2002
Strains with modifications: 158
Nocardioides ginsengagri DSM 21362

+  1 ml Modified Wolin's mineral solution
+  0.01 ml Trace element solution SL-10

Media similar to medium 830

1153 R3 A MEDIUM
1492 HALF STRENGTH R2A
1578 1/10 R2A MEDIUM

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Glucose 0.5 2.775
Casamino acids 0.5 -
Starch 0.5 -
Proteose peptone 0.5 -
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Cultivation metadata from 2002 strains BacDive

halophily non-halophilic 17 strains
halophily moderately halophilic 2 strains
pH 6 - 8 499 strains
pH 4 - 6 264 strains
pH 8 - 10 249 strains
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