830
830 .
R2A MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Azospira oryzae DSM 11199
* Modification: Aerobic; reactivate lyophilized cells in liquid medium 830, incubate for at least 10 days
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 28 °C
Main sol. 830 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(BD 212750) |
0.50 | g | - | - | |
|
Proteose peptone
(Difco no. 3) |
0.50 | g | - | - | |
| Casamino acids | 0.50 | g | - | - | |
| Glucose | 0.50 | g | - | - | |
|
Starch
(soluble) |
0.50 | g | - | - | |
| Na-pyruvate | 0.30 | g | - | - | |
| K2HPO4 | 0.30 | g | - | - | |
| MgSO4 x 7 H2O | 0.05 | g | - | - | |
| Agar | 15.00 | g | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C. | |||||
| 2 Note: this equals BD 218263 | |||||
Metadata
| Last modified: | 23.05.25 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Glucose | 0.5 | 2.775 |
| Casamino acids | 0.5 | - |
| Starch | 0.5 | - |
| Proteose peptone | 0.5 | - |
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Cultivation metadata from 2002 strains
| halophily | non-halophilic | 17 strains |
| halophily | moderately halophilic | 2 strains |
| pH | 6 - 8 | 499 strains |
| pH | 4 - 6 | 264 strains |
| pH | 8 - 10 | 249 strains |
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