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DSMZ

DSMZ 81a . THIOMONAS ISLANDICA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 81a #

ml
Equipment needed: Gassing station Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 2.30 g 2.275 13.061
Na2HPO4 x 2 H2O 2.90 g 2.868 20.206
NH4Cl 1.00 g 0.989 18.492
CaCl2 x 2 H2O 0.01 g 0.01 0.067
MgSO4 x 7 H2O 0.50 g 0.495 2.007
NaVO3 x H2O
(0.1% w/v) 		5.00 ml 0.005 0.035
Trace element solution SL-6 5.00 ml - -
NaHCO3 0.50 g 0.495 5.887
Ferric ammonium citrate 0.05 g 0.049 -
Seven vitamins solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (ecxept bicarbonate, ferric ammonium citrate and vitamins), then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Adjust pH to 4.5 and dispense solution under air atmosphere into serum vials suitable for anaerobic cultivation to 50% of volume. Close vials with butyl rubber septa to prevent free exchange of oxygen with the external atmosphere and autoclave. Add ferric ammonium citrate and vitamins from anoxic stock solutions sterilized by filtration and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of the complete medium to 5.0, if necessary.
2 After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure.
3 Note: Use at least 10% (v/v) as inoculum.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 27.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 5.0
Equipment needed: Autoclave, Filter, Gassing station
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Gas composition

  • 53 % H2
  • 27 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 2.868 20.206
K2HPO4 2.275 13.061
NH4Cl 0.989 18.492
NaHCO3 0.495 5.887
MgSO4 x 7 H2O 0.495 2.007
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
oxygen microaerophile 1 strain
temperature thermophilic 1 strain