MediaDive DSMZ Digital Diversity
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder SPARQL endpoint Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 81 . MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Hydrogenobacter thermophilus DSM 6534

* Modification: Incubate under 5% (v/v) O2, 45% H2, 40% N2, 10% CO2.

Growth conditions: 3-7 days 70 °C

Main sol. 81 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 50 ml - -
Solution B 925 ml - -
Solution C 20 ml - -
Standard vitamin solution 5 ml - -
1 Solutions A, B, C are autoclaved separately for 15 min at 121°C, cooled down to 50°C and then mixed aseptically with 5.0 ml filter-sterilized standard vitamin solution (see below).The final pH of this medium should be 6.8 without adjustment.
2 For chemolithotrophic growth incubate the culture under an atmosphere of 2% (v/v) O2, 10% CO2, 60% H2 and 28% N2. For heterotrophic growth supplement the mineral medium with an appropriate carbon source (0.2% carbohydrate or 0.1% organic acid). For growth on nitrogen-free medium, omit NH4Cl and incubate the culture under an atmosphere of 2% (v/v) O2, 10% CO2, 10% H2 and 78% N2 or heterotrophically under 2% (v/v) O2 and 98% N2. For more details see Ref. 1515 and Ref. 3363.

Solution A #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 2.3 g 46 338.022
Na2HPO4 x 2 H2O 2.9 g 58 408.569
Distilled water 50.0 ml - -

Solution B #

925
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 1.087 20.321
MgSO4 x 7 H2O 0.50 g 0.543 2.205
CaCl2 x 2 H2O 0.01 g 0.011 0.074
MnCl2 x 4 H2O 5.00 mg 0.005 -
NaVO3 x H2O 5.00 mg 0.005 0.039
Trace element solution SL-6 5.00 ml - -
Distilled water 920.00 ml - -
Agar , if required 20.00 g 21.739 64.636

Solution C #

20
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ferric ammonium citrate 0.05 g 2.5 9.543
Distilled water 20.00 ml - -

Standard vitamin solution #

100
Solution occurs in 3 other media (first defined in Medium 81).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Riboflavin 10.0 mg 0.1 0.266
Thiamine-HCl x 2 H2O 50.0 mg 0.5 1.339
Nicotinic acid 50.0 mg 0.5 4.061
Pyridoxine hydrochloride 50.0 mg 0.5 2.431
Calcium pantothenate 50.0 mg 0.5 1.049
Biotin 0.1 mg 1.0e-3 0.004
Folic acid 0.2 mg 0.002 0.005
Vitamin B12 1.0 mg 0.01 0.007
Distilled water 100.0 ml - -

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 01.10.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Autoclave, Filter
Edit in Medium builder

Gas composition

  • 45 % H2
  • 40 % N2
  • 10 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 125
Strains with modifications: 6
Hydrogenobacter thermophilus DSM 6534

  45% H2, 40% N2, 10% CO2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 20.101 59.764
Na2HPO4 x 2 H2O 2.915 20.531
KH2PO4 2.312 16.986
NH4Cl 1.005 18.789
MgSO4 x 7 H2O 0.503 2.039
Show more
CSV
JSON

Cultivation metadata from 125 strains BacDive

pH 6 - 8 3 strains
pH 10 - 12 2 strains
phyla 3 126 strains
nutrition chemolithoautotroph 3 strains
nutrition chemoorganotroph 3 strains
Show more