815a
815a .
DESULFOCELLA HALOPHILA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 815a #
ml
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 3.0 | g | 3 | 21.121 | |
| CaCl2 x 2 H2O | 6.0 | g | 6 | 40.812 | |
| NaCl | 40.0 | g | 40 | 684.463 | |
| KCl | 2.0 | g | 2 | 26.828 | |
| NH4Cl | 0.3 | g | 0.3 | 5.609 | |
| KH2PO4 | 0.2 | g | 0.2 | 1.47 | |
| MgCl2 x 6 H2O | 8.0 | g | 8 | 39.35 | |
| SrCl2 x 6 H2O | 0.1 | g | 0.1 | 0.375 | |
| Trace element solution SL-11 | 1.0 | ml | - | - | |
| MOPS | 3.0 | g | 3 | 14.336 | |
| Yeast extract | 1.0 | g | 1 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| Na-pyruvate | 2.5 | g | 2.5 | 22.718 | |
| Na2CO3 | 0.5 | g | 0.5 | 4.718 | |
| Na2S x 9 H2O | 0.1 | g | 0.1 | 0.416 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except pyruvate, bicarbonate and sulfide, adjust pH to 6.0 with NaOH and sparge medium for 30 - 45 min with 100% N2 gas to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add pyruvate (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Final medium pH should be 7.2. | |||||
| 2 Note: Prior to inoculation 10-20 mg/l sodium dithionite (added from a 5% w/v solution freshly prepared under N2 and filter-sterilized) can be added to the medium to stimulate growth at the beginning. | |||||
Solution occurs in 22
other media (first defined in Medium 722).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA x 2 H2O | 5.2 | g | 5.2 | 15.467 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml. | |||||
Metadata
| Last modified: | 07.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 40 | 684.463 |
| MgCl2 x 6 H2O | 8 | 39.35 |
| CaCl2 x 2 H2O | 6 | 40.812 |
| Na2SO4 | 3 | 21.121 |
| MOPS | 3 | 14.336 |
| Show more | ||
