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DSMZ

DSMZ 813b . DESULFONATRONUM LACUSTRE MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 813b #

ml
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.2 g 0.199 1.465
MgCl2 x 6 H2O 0.1 g 0.1 0.49
NH4Cl 1.0 g 0.997 18.639
KCl 0.2 g 0.199 2.675
Na2SO4 5.0 g 4.985 35.096
NaCl 2.0 g 1.994 34.121
Trace element solution SL-10 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Yeast extract 1.0 g 0.997 -
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.0e-4 0.002
Na2CO3 3.5 g 3.49 32.924
Na2S x 9 H2O 0.5 g 0.499 2.076
Na-L-lactate 2.0 g 1.994 -
Wolin's vitamin solution (10x) 1.0 ml - -
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except carbonate, sulfide, lactate, and vitamins) and sparge medium with 100 % N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate and sulfide, then adjust pH to 8.8 - 9.0, dispense under 100% N2 gas atmosphere into Hungate-type tubes or serum vials and autoclave. Before use add lactate and vitamins (sterilized by filtration) from sterile anoxic stock solutions prepared under 100% N2 gas.

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 178 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 13.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.8 - 9.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 4
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2SO4 4.985 35.096
Na2CO3 3.49 32.924
NaCl 1.994 34.121
Na-L-lactate 1.994 -
NH4Cl 0.997 18.639
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Cultivation metadata from 4 strains BacDive

halophily halotolerant 1 strain
pH 8 - 10 1 strain
pH 10 - 12 1 strain
phyla 1 3 strains
oxygen anaerobe 3 strains
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