80
80 .
GLYCEROL-SOIL MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 80 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | 5 | - | |
| Beef extract | 3 | g | 3 | - | |
| Glycerol | 20 | g | 20 | 217.179 | |
| Soil extract | 150 | ml | - | - | |
| Distilled water | 850 | ml | - | - | |
| Agar | 15 | g | 15 | 44.599 | |
| 1 Adjust pH to 7.0. Soil extract is prepared by sieving air dried garden soil through a coarse sieve and autoclaving 400 g with 960 ml of distilled water at 121°C for one hour. After the mixture is cool and settled, the supernatant is carefully decanted, filtered through paper, autoclaved in 200 ml quantities, and stored at room temperature until cleared by sedimentation. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 14 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glycerol | 20 | 217.179 |
| Agar | 15 | 44.599 |
| Peptone | 5 | - |
| Beef extract | 3 | - |
| Distilled water | - | - |
| Show more | ||
Cultivation metadata from 14 strains
| phyla | 1 | 15 strains |
| oxygen | obligate aerobe | 6 strains |
| oxygen | aerobe | 1 strain |
| temperature | mesophilic | 15 strains |