MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 792b . CAMINIBACTER MEDIATLANTICUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 792b #

ml
Equipment needed: Gassing station Serum bottle Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 989 ml - -
Solution B 10 ml - -
Solution C 10 ml - -
1 Sparge solution A with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic serum vials (e.g., 20 ml medium in 100 ml bottles) and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas mixture and solution C is autoclaved under 100% N2 gas. To complete the medium appropriate amounts of solutions B and C are added to solution A. Adjust pH of the complete medium to 5.7.
2 After inoculation pressurize vials to 2 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
3 Note: Solution A has to be prepared freshly. It cannot be stored for a long period of time.

Solution A #

989
Equipment needed: Gassing station Autoclave Serum bottle
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 30.334 519.057
MgSO4 x 7 H2O 3.50 g 3.539 14.358
MgCl2 x 6 H2O 2.75 g 2.781 13.677
CaCl2 x 2 H2O 0.75 g 0.758 5.158
KCl 0.33 g 0.334 4.476
NaBr 0.05 g 0.051 0.491
H3BO3 15.00 mg 0.015 0.245
SrCl2 x 6 H2O
(0.1% w/v) 		7.00 ml 0.007 0.027
KI
(0.01% w/v) 		0.50 ml 5.1e-5 1.9e-4
Wolfe's mineral elixir 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.1e-4 0.002
Distilled water 980.00 ml - -

Solution B #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
KNO3 1.0 g 100 989.09
KH2PO4 0.5 g 50 367.417
Distilled water 10.0 ml - -

Solution C #

10
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.15 g 15 62.453
Distilled water 10.00 ml - -

Wolfe's mineral elixir #

1000
Solution occurs in 21 other media (first defined in Medium 792).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x H2O 5.00 g 5 29.583
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 0.379
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.

Metadata

Last modified: 17.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 5.7
Equipment needed: Autoclave, Gassing station, Serum bottle
Edit in Medium builder

Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.725 13.406
KNO3 0.991 9.803
KH2PO4 0.496 3.641
KCl 0.327 4.387
Na2S x 9 H2O 0.149 0.619
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain