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DSMZ

DSMZ 766 . THERMOCOCCUS CHITINOPHAGUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 766 #

ml
Equipment needed: Hungate tubes Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.50 g 0.5 3.784
NaHCO3 0.20 g 0.2 2.381
KH2PO4 0.50 g 0.5 3.674
Na2WO4 x 2 H2O 0.15 mg 1.5e-4 4.5e-4
Na2SeO4 0.15 mg 1.5e-4 7.9e-4
(NH4)2Ni(SO4)2 0.30 mg 3.0e-4 7.6e-4
Chitin stock solution 4.00 g 4 -
Na2S x 9 H2O 0.50 g 0.5 2.082
Modified Wolin's mineral solution II 15.00 ml - -
Resazurin 1.00 mg 1.0e-3 0.004
Artifical sea water
(synthetic) 		485.00 ml - -
Distilled water 500.00 ml - -
1 Prepare the medium without Na2S x 9 H2O and NaHCO3, boil and cool under N2. Add the NaHCO3 to the cooled medium and adjust the pH to 6.7. Dispense into Hungate tubes or serum bottles under N2 and autoclave. Reduce the medium before use from a sterile stock solution of neutralised Na2S x 9 H2O.

Chitin stock solution #

1200
Compound Amount Unit Conc. [g/L] Conc. [mM]
Chitin 20 g 16.667 -
HCl
(37 %) 		200 ml 74 2029.62
Distilled water 1000 ml - -
1 The chitin solution is prepared as follows: mix 20 g chitin (practical grade from crab shells) with 200 ml of 37% HCl (pre-cooled to 4°C) and stir for 1 hr at 4°C. Pour the suspension into 1 litre of distilled water (pre-cooled to 4°C) and filter through filter paper (Schleicher and Schüll Nr. 311853). Wash the residue five times with 500 ml distilled water and resuspend in 1 litre of distilled water. Neutralise the suspension with 10 ml of 5M KOH (final pH 6.5). Filter and wash with 3 litres of distilled water to remove KCl.

Artifical sea water #

1000
Solution occurs in 2 other media (first defined in Medium 600).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 23.47 g 23.47 401.608
Na2SO4 3.92 g 3.92 27.598
MgCl2 x 6 H2O 10.64 g 10.64 52.336
CaCl2 1.10 g 1.1 9.912
NaHCO3 192.00 mg 0.192 2.286
KCl 664.00 mg 0.664 8.907
KBr 96.00 mg 0.096 0.807
H3BO3 26.00 mg 0.026 0.42
SrCl2 24.00 mg 0.024 0.151
NaF 3.00 mg 0.003 0.071
Distilled water 1000.00 ml - -

Modified Wolin's mineral solution II #

1000
Solution occurs in 23 other media (first defined in Medium 700).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
MgSO4 x 7 H2O 3.000 g 3 12.172
MnSO4 x H2O 0.500 g 0.5 2.958
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CoSO4 x 7 H2O 0.180 g 0.18 1.161
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnSO4 x 7 H2O 0.180 g 0.18 0.626
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.020 g 0.02 0.042
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Na2SeO3 x 5 H2O 0.300 mg 3.0e-4 0.001
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 6.7
Equipment needed: Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 11.383 194.78
MgCl2 x 6 H2O 5.16 25.383
Na2SO4 1.901 13.385
CaCl2 0.534 4.807
(NH4)2SO4 0.5 3.784
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain