760
760 .
MARINE THERMOCOCCUS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Thermococcus sibiricus DSM 12597
* Modification: Anaerobic gas mixture N2, pH 7.5
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 78 °C
Main sol. 760 #
ml
Equipment needed: Anaerobic workbench
Hungate tubes
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium 514 | 1000 | ml | - | - | |
| 1 Prepare liquid Bacto Marine Broth (see medium 514) and filter through normal filter paper. An iron sediment will collect in the filter. Boil the medium in a water bath and cool under an anaerobic gas mixture (see strain dependant modifications). | |||||
| 2 Dispense the medium into Hungate tubes or serum bottles containing finely divided sulphur (0.5% w/v), seal the tubes or bottles under the same anaerobic gas used when cooling the medium. Sterilise the medium at 100°C for 3 hours on 3 consecutive days. Reduce the medium by adding 10% neutralised sodium sulphide to a final concentration of 0.05 %. The medium should not give a heavy black precipitate, if it does the iron sediment was not adequately removed by filtering in the initial stages and the medium should be made again, making sure that the iron is removed by filtering. | |||||
Solution occurs in 4
other media (first defined in Medium 514).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Bacto peptone | 5.00 | g | 5 | - | |
|
Yeast extract
(Bacto ) |
1.00 | g | 1 | - | |
| Fe(III) citrate | 0.10 | g | 0.1 | 0.408 | |
| NaCl | 19.45 | g | 19.45 | 332.82 | |
|
MgCl2
(anhydrous) |
5.90 | g | 5.9 | 61.968 | |
| Na2SO4 | 3.24 | g | 3.24 | 22.811 | |
| CaCl2 | 1.80 | g | 1.8 | 16.219 | |
| KCl | 0.55 | g | 0.55 | 7.378 | |
| NaHCO3 | 0.16 | g | 0.16 | 1.905 | |
| KBr | 0.08 | g | 0.08 | 0.672 | |
| SrCl2 | 34.00 | mg | 0.034 | 0.214 | |
| H3BO3 | 22.00 | mg | 0.022 | 0.356 | |
| Na-silicate | 4.00 | mg | 0.004 | 0.033 | |
| NaF | 2.40 | mg | 0.002 | 0.057 | |
| (NH4)NO3 | 1.60 | mg | 0.002 | 0.02 | |
| Na2HPO4 | 8.00 | mg | 0.008 | 0.056 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.40 g to 1 litre water. | |||||
Metadata
| Last modified: | 19.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | * 7.5 |
| Equipment needed: | Anaerobic workbench, Filter, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 19.45 | 332.82 |
| MgCl2 | 5.9 | 61.968 |
| Bacto peptone | 5 | - |
| Na2SO4 | 3.24 | 22.811 |
| CaCl2 | 1.8 | 16.219 |
| Show more | ||
Cultivation metadata from 32 strains
| phyla | 1 | 33 strains |
| oxygen | anaerobe | 23 strains |
| temperature | hyperthermophilic | 29 strains |
| temperature | thermophilic | 2 strains |