756d .
NITROSPIRA MOSCOVIENSIS
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Equipment needed:
Final pH:
7.4 - 7.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trace element solution | 1.0 | ml | ||
|
|
Stock solution | 100.0 | ml | ||
|
|
NaNO2 | 0.5 | g | ||
|
|
Distilled water | 899.0 | ml | ||
| 1 Adjust pH to 8.6 with NaOH or KOH. Autoclave and leave the medium to stand for 2 - 3 days so that pH can ajust itself to pH 7.4 - 7.6 . | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
MnSO4 x H2O | 33.8 | mg |
|
|
H3BO3 | 49.4 | mg |
|
|
ZnSO4 x 7 H2O | 43.1 | mg |
|
|
(NH4)6Mo7O24 | 37.1 | mg |
|
|
FeSO4 x 7 H2O | 97.3 | mg |
|
|
CuSO4 x 5 H2O | 25.0 | mg |
|
|
Distilled water | 1000.0 | ml |
Stock solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
CaCO3 | 0.07 | g |
|
|
NaCl | 5.00 | g |
|
|
MgSO4 x 7 H2O | 0.50 | g |
|
|
KH2PO4 | 1.50 | g |
|
|
Distilled water | 1000.00 | ml |