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DSMZ

DSMZ 756d . NITROSPIRA MOSCOVIENSIS

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Nitrospira defluvii DSM 118082

* Modification: Use 0.02 g/l NaNO2. Use 100 - 150 ml medium in 300 ml Erlenmeyer flasks with metal caps. The medium has to be prepared 2-3 days before inoculation. The final pH should be 7.4-7.6. Inoculate 1 ml per flask and incubate the culture in the dark without stirring. It might take several weeks until the nitrite is consumed. Use a spot test for presence of nitrite on filter paper (e.g., MQuant Nitrite Test, Sigmaldrich 1.10007.0001).

Growth conditions: more than 14 days aerobic 28 °C

Main sol. 756d #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution 1.00 ml - -
Stock solution 100.00 ml - -
NaNO2* 0.02 g/l - -
Distilled water 899.00 ml - -
1 Adjust pH to 8.6 with NaOH or KOH. Autoclave and leave the medium to stand for 2 - 3 days so that pH can ajust itself to pH 7.4 - 7.6 .

Trace element solution #

1000
Solution occurs in 4 other media (first defined in Medium 756).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnSO4 x H2O 33.8 mg 0.034 0.2
H3BO3 49.4 mg 0.049 0.799
ZnSO4 x 7 H2O 43.1 mg 0.043 0.15
(NH4)6Mo7O24 37.1 mg 0.037 -
FeSO4 x 7 H2O 97.3 mg 0.097 0.35
CuSO4 x 5 H2O 25.0 mg 0.025 0.1
Distilled water 1000.0 ml - -

Stock solution #

1000
Solution occurs in 4 other media (first defined in Medium 756).
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCO3 0.07 g 0.07 0.699
NaCl 5.00 g 5 85.558
MgSO4 x 7 H2O 0.50 g 0.5 2.029
KH2PO4 1.50 g 1.5 11.023
Distilled water 1000.00 ml - -

Metadata

Last modified: 24.03.22
Source: DSMZ
Medium type: Defined medium
Final pH: 7.4 - 7.6
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Associated strains

All strains for this medium: 2
Strains with modifications: 2
Nitrospira defluvii DSM 118082

  NaNO2
  0.02 g/l NaNO2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 0.5 8.556
NaNO2 0.5 7.247
KH2PO4 0.15 1.102
MgSO4 x 7 H2O 0.05 0.203
CaCO3 0.007 0.07
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains