743
743 .
HYDROGENOBACTER ACIDOPHILUS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Hydrogenobaculum acidophilum DSM 11251
* Modification: Gas phase H2:O2:CO2=7:1:1
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 1-2 days
65 °C
Main sol. 743 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Sulfur | 5.0 | g | 5 | 155.909 | |
| (NH4)2SO4 | 1.0 | g | 1 | 7.568 | |
| K2HPO4 | 1.0 | g | 1 | 5.741 | |
| NaCl | 1.0 | g | 1 | 17.112 | |
| MgSO4 x 7 H2O | 0.3 | g | 0.3 | 1.217 | |
| FeSO4 x 7 H2O | 1.0 | mg | 1.0e-3 | 0.004 | |
| CaCl2 | 1.0 | mg | 1.0e-3 | 0.009 | |
| Trace element solution | 0.5 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 3.0 with HCl. Steam medium for 3 hours on each of 3 successive days. Incubate under a gas phase H2:O2:CO2 = 7:1:1 with an overpressure of one atmosphere at 65°C. | |||||
Solution occurs in 2
other media (first defined in Medium 533).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
H2O
(deionized) |
1000 | ml | - | - |
| MoO3 | 4 | mg | 0.004 | 0.028 |
| ZnSO4 x 7 H2O | 28 | mg | 0.028 | 0.097 |
| CuSO4 x 5 H2O | 2 | mg | 0.002 | 0.008 |
| H3BO3 | 4 | mg | 0.004 | 0.065 |
| MnSO4 x 5 H2O | 4 | mg | 0.004 | 0.017 |
| CoCl2 x 6 H2O | 4 | mg | 0.004 | 0.031 |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 3.0 |
| Edit in Medium builder | |
Gas composition
- 77.7 % H2
- 11.1 % CO2
- 11.1 % O2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 5 | 155.909 |
| NaCl | 1 | 17.112 |
| (NH4)2SO4 | 1 | 7.568 |
| K2HPO4 | 1 | 5.741 |
| MgSO4 x 7 H2O | 0.3 | 1.217 |
| Show more | ||
