712
712 .
PSEUDOBUTYRIVIBRIO MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 712 #
ml
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 0.23 | g | 0.23 | 1.319 | |
| KH2PO4 | 0.23 | g | 0.23 | 1.688 | |
| NaCl | 0.45 | g | 0.45 | 7.693 | |
| (NH4)2SO4 | 0.45 | g | 0.45 | 3.402 | |
| CaCl2 x 2 H2O | 0.06 | g | 0.06 | 0.408 | |
| MgSO4 x 7 H2O | 0.09 | g | 0.09 | 0.365 | |
| Yeast extract | 5.00 | g | 4.995 | - | |
| Indigocarmine | 5.00 | mg | 0.005 | 0.011 | |
| NaHCO3 | 6.40 | g | 6.394 | 76.108 | |
| D-Glucose | 5.00 | g | 4.995 | 27.725 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.30 | g | 0.3 | 1.706 | |
| Na2S x 9 H2O | 0.30 | g | 0.3 | 1.248 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate, vitamins, glucose, cysteine and sulfide), then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add the bicarbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute medium under CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add vitamins, glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins are sterilized by filtration. Adjust pH of complete medium to 6.7 - 6.8, if necessary. | |||||
Solution occurs in 308
other media (first defined in Medium 120).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 20 | mg | 0.02 | 0.082 |
| Folic acid | 20 | mg | 0.02 | 0.045 |
| Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
| Thiamine HCl | 50 | mg | 0.05 | 0.148 |
| Riboflavin | 50 | mg | 0.05 | 0.133 |
| Nicotinic acid | 50 | mg | 0.05 | 0.406 |
| Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
| Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
| p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
| (DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 06.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.7 - 6.8 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaHCO3 | 6.394 | 76.108 |
| D-Glucose | 4.995 | 27.725 |
| Yeast extract | 4.995 | - |
| NaCl | 0.45 | 7.693 |
| (NH4)2SO4 | 0.45 | 3.402 |
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