689
689 .
ACE MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 689 #
ml
Equipment needed: Autoclave
Gassing station
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KCl | 0.33 | g | 0.329 | 4.413 | |
| MgCl2 x 6 H2O | 6.00 | g | 5.982 | 29.424 | |
| MgSO4 x 7 H2O | 1.00 | g | 0.997 | 4.045 | |
| NH4Cl | 0.25 | g | 0.249 | 4.66 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.14 | 0.949 | |
| K2HPO4 | 0.14 | g | 0.14 | 0.801 | |
| NaCl | 18.00 | g | 17.946 | 307.087 | |
|
Fe(NH4)2(SO4)2 x 6 H2O
(0.1% w/v) |
2.00 | ml | 0.002 | - | |
|
Yeast extract
(Difco) |
1.00 | g | 0.997 | - | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.499 | 2.838 | |
| NaHCO3 | 2.00 | g | 1.994 | 23.736 | |
| Glucose | 10.00 | g | 9.97 | 55.34 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except cysteine, bicarbonate, glucose and vitamins, then sparge medium with 100% N2 gas to make it anoxic. Add cysteine and sodium bicarbonate, then adjust pH to 7.5. Dispense medium in anoxic Hungate-type tubes under 100% N2 gas and autoclave. A precipitate forms which will redissolve slowly over about 3 days. Before use inject separately sterile glucose solution (20% w/v) and vitamins prepared under 100% N2 gas atmosphere. Vitamins should be sterilized by filtration. Check medium pH and adjust pH to 7.6, if necessary. | |||||
Solution occurs in 308
other media (first defined in Medium 120).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 20 | mg | 0.02 | 0.082 |
| Folic acid | 20 | mg | 0.02 | 0.045 |
| Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
| Thiamine HCl | 50 | mg | 0.05 | 0.148 |
| Riboflavin | 50 | mg | 0.05 | 0.133 |
| Nicotinic acid | 50 | mg | 0.05 | 0.406 |
| Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
| Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
| p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
| (DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 11.10.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.6 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 17.946 | 307.087 |
| Glucose | 9.97 | 55.34 |
| MgCl2 x 6 H2O | 5.982 | 29.424 |
| NaHCO3 | 1.994 | 23.736 |
| MgSO4 x 7 H2O | 0.997 | 4.045 |
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