Name: HALOTHIOBACILLUS NEAPOLITANUS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 68 #

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	4.0	g	4	29.393
K₂HPO₄	4.0	g	4	22.965
MgSO₄ x 7 H₂O	0.8	g	0.8	3.246
NH₄Cl	0.4	g	0.4	7.478
Trace element solution (Vishniac & Santer, 1957)	5.0	ml	-	-
Bromocresol purple (saturated aqueous solution)	0.5	ml	-	-
Agar , for solid medium (Bacto) optional	15.0	g	15	44.599
Na₂S₂O₃ x 5 H₂O	10.0	g	10	40.292
Distilled water	1000.0	ml	-	-
1 Dissolve ingredients except thiosulfate, dispense into suitable cultivation vessels and autoclave. Add thiosulfate to the autoclaved medium from a sterile stock solution. Adjust pH of complete medium to 6.6 - 7.0.

Trace element solution (Vishniac & Santer, 1957) #

1000

Solution occurs in 4 other media (first defined in Medium 69).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂-EDTA	50.00	g	50	148.718
ZnSO₄ x 7 H₂O	22.00	g	22	76.506
CaCl₂ x 2 H₂O	5.54	g	5.54	37.683
MnCl₂ x 4 H₂O	5.06	g	5.06	25.568
FeSO₄ x 7 H₂O	5.00	g	5	17.985
(NH₄)₆Mo₇O₂₄ x 4 H₂O	1.10	g	1.1	0.89
CuSO₄ x 5 H₂O	1.57	g	1.57	6.288
CoCl₂ x 6 H₂O	1.61	g	1.61	12.4
Distilled water	1000.00	ml	-	-
1 Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.

All strains for this medium:

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