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DSMZ

DSMZ 655 . PYE MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 655 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 1.00 g 1 5.741
MgSO4 x 7 H2O 0.20 g 0.2 0.811
CaCl2 x 2 H2O 0.02 g 0.02 0.136
Na2S2O3 x 5 H2O 0.20 g 0.2 0.806
Na-pyruvate 2.20 g 2.2 19.992
Yeast extract
(Difco) 		4.00 g 4 -
(NH4)2SO4 1.00 g 1 7.568
Trace element solution SL-6 1.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 6.8.
2 Boil the medium for few minutes. Fill in tubes with rubber stoppers under nitrogen gas. Sterilize at 121°C for 15 min.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
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Associated strains

All strains for this medium: 3
Strains with modifications: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 4 -
Na-pyruvate 2.2 19.992
(NH4)2SO4 1 7.568
K2HPO4 1 5.741
MgSO4 x 7 H2O 0.2 0.811
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen anaerobe 3 strains
oxygen obligate anaerobe 1 strain
temperature mesophilic 2 strains
temperature thermophilic 1 strain