652
652 .
HALOPHILE MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 652 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 100 | g | 100 | 1711.16 | |
| KCl | 5 | g | 5 | 67.069 | |
| MgCl2 x 6 H2O | 5 | g | 5 | 24.594 | |
| NH4Cl | 5 | g | 5 | 93.475 | |
| MgSO4 x 7 H2O | 5 | g | 5 | 20.286 | |
| Yeast extract | 5 | g | 5 | - | |
|
Peptone
(Difco) |
5 | g | 5 | - | |
| Trace element solution | 5 | ml | - | - | |
|
Fe(III) citrate
(1%) |
5 | ml | 0.05 | 0.204 | |
| Distilled water | 990 | ml | - | - | |
| 1 Adjust pH to 7.5-8.0. Boil the medium for few minutes. Fill in tubes with rubber stoppers under nitrogen gas. Sterilize at 121°C for 15 min. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CuSO4 x 5 H2O | 1.0 | mg | 1.0e-3 | 0.004 |
| ZnSO4 x 7 H2O | 220.0 | mg | 0.22 | 0.765 |
| CoCl2 x 6 H2O | 10.0 | mg | 0.01 | 0.077 |
| MnCl2 x 4 H2O | 180.0 | mg | 0.18 | 0.91 |
| Na2MoO4 x H2O | 6.3 | mg | 0.006 | 0.031 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 - 8.0 |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 100 | 1711.16 |
| NH4Cl | 5 | 93.475 |
| KCl | 5 | 67.069 |
| MgCl2 x 6 H2O | 5 | 24.594 |
| MgSO4 x 7 H2O | 5 | 20.286 |
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