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DSMZ

DSMZ 641 . DESULFOVIBRIO (MV) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Thermodesulfovibrio hydrogeniphilus DSM 18151

* Modification: Replace lactate with 0.2 g/l Na-acetate. After inoculation pressurize culture vessels to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.

Growth conditions: anaerobic 65 °C

Main sol. 641 #

ml
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 0.997 18.639
Na2SO4 2.0 g 1.994 14.038
Na2S2O3 x 5 H2O 1.0 g 0.997 4.017
MgSO4 x 7 H2O 1.0 g 0.997 4.045
CaCl2 x 2 H2O 0.1 g 0.1 0.678
KH2PO4 0.5 g 0.499 3.663
Trace element solution SL-10 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Yeast extract 1.0 g 0.997 -
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.0e-4 0.002
Na2CO3 1.0 g 0.997 9.407
Na-DL-lactate* 2.5 g 2.493 22.243
Wolin's vitamin solution (10x) 1.0 ml - -
Na2S x 9 H2O 0.1 g 0.1 0.415
Na acetate* 0.2 g - -
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except carbonate, vitamins, lactate and sulfide), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving complete the medium by adding vitamins (sterilized by filtration), lactate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of the complete medium to 7.0 - 7.2, if necessary.
2 Note: Prior to inoculation 10-20 mg/l sodium dithionite (added from a 5% w/v solution freshly prepared under N2 and filter-sterilized) can be added to the medium to stimulate growth at the beginning.

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 179 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Wolin's vitamin solution (10x) #

1000
Solution occurs in 306 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 16.08.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 61
Strains with modifications: 19
Thermodesulfovibrio hydrogeniphilus DSM 18151

  80% H2, 20% CO2
  Na-DL-lactate
  0.2 g Na acetate

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Na2SO4 1.994 14.038
NH4Cl 0.997 18.639
Na2CO3 0.997 9.407
MgSO4 x 7 H2O 0.997 4.045
Na2S2O3 x 5 H2O 0.997 4.017
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Cultivation metadata from 61 strains BacDive

pH 6 - 8 18 strains
pH 8 - 10 10 strains
pH 4 - 6 5 strains
phyla 4 58 strains
nutrition chemolithoautotroph 2 strains
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