MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 63 . DESULFOVIBRIO (POSTGATE) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Desulfovibrio diazotrophica DSM 109475

* Modification: Adjust pH of complete medium to 6.0-6.5.

Growth conditions: anaerobic 37 °C

Main sol. 63 #

ml
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 980 ml - -
Solution B 10 ml - -
Solution C 10 ml - -
1 Dissolve ingredients of solution A, bring to the boil, then cool to room temperature while sparging with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under 100% N2 gas atmosphere into anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121 °C. Adjust pH of the complete medium to 6.8 - 7.0, if necessary.

Solution A #

980
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.5 g 0.51 2.929
NH4Cl 1.0 g 1.02 19.077
Na2SO4 1.0 g 1.02 7.184
CaCl2 x 2 H2O 0.1 g 0.102 0.694
MgSO4 x 7 H2O 2.0 g 2.041 8.28
Na-DL-lactate 2.0 g 2.041 18.212
Yeast extract 1.0 g 1.02 -
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.1e-4 0.002
Distilled water 980.0 ml - -

Solution B #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 0.5 g 50 179.846
Distilled water 10.0 ml - -

Solution C #

10
Equipment needed: Hungate tubes Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-thioglycolate 0.1 g 10 87.643
Ascorbic acid 0.1 g 10 56.78
Distilled water 10.0 ml - -

Metadata

Last modified: 05.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: * 6.0 - 6.5
Equipment needed: Autoclave, Gassing station, Hungate tubes
Edit in Medium builder

Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 91
Strains with modifications: 2
Desulfovibrio diazotrophica DSM 109475

  6.0 - 6.5

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na-DL-lactate 2 17.848
MgSO4 x 7 H2O 2 8.114
NH4Cl 1 18.695
Na2SO4 1 7.04
Yeast extract 1 -
Show more
CSV
JSON

Cultivation metadata from 91 strains BacDive

pH 6 - 8 7 strains
pH 8 - 10 3 strains
pH 4 - 6 2 strains
pH 2 - 4 1 strain
phyla 4 87 strains
Show more