MediaDive DSMZ Digital Diversity
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder SPARQL endpoint Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 621a . EKHO LAKE STRAINS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 621a #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral salt solution
("Hutner/Cohen-Bazire") 		20.00 ml - -
Peptone
(Bacto) 		0.25 g 0.25 -
Yeast extract
(Bacto) 		0.25 g 0.25 -
Agar
(Bacto) 		15.00 g 15 44.599
Artifical sea water 965.00 ml - -
1 Sterilize 20 min./121°C. After cooling to 60°C add to the medium:
Glucose
(2.5%, sterile-filtered) 		10.00 ml 0.25 1.388
Vitamin solution (double conc.) 5.00 ml - -
2 The percentage Artificial Sea Water (ASW) indicates the percentage ratio of distilled water to artifical sea water (i.e. "medium 621a + 25ASW" = medium 621 made with 750 ml distilled water + 250 ml ASW). The pH is 7.2 - 7.4.

Artifical sea water #

1000
Solution occurs in 2 other media (first defined in Medium 607).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 23.477 g 23.477 401.728
Na2SO4 3.917 g 3.917 27.577
MgCl2 x 6 H2O 4.981 g 4.981 24.5
CaCl2 1.102 g 1.102 9.93
NaHCO3 192.000 mg 0.192 2.286
KCl 664.000 mg 0.664 8.907
KBr 6.000 mg 0.006 0.05
H3BO3 26.000 mg 0.026 0.42
SrCl2 24.000 mg 0.024 0.151
NaF 3.000 mg 0.003 0.071
Distilled water 1000.000 ml - -

Mineral salt solution #

950
Solution occurs in 3 other media (first defined in Medium 621).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		10.00 g 10.526 55.071
MgSO4 x 7 H2O 29.70 g 31.263 126.841
CaCl2 x 2 H2O 3.34 g 3.516 23.915
Na2MoO4 x 2 H2O 12.67 mg 0.013 0.055
FeSO4 x 7 H2O 99.00 mg 0.104 0.375
Metall salt sol. 44
(see medium 590) 		50.00 ml - -
Distilled water 900.00 ml - -
1 Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water.

Vitamin solution (double conc.) #

1000
Solution occurs in 6 other media (first defined in Medium 621).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.0 mg 0.004 0.016
Folic acid 4.0 mg 0.004 0.009
Pyridoxine hydrochloride 20.0 mg 0.02 0.097
Riboflavine 10.0 mg 0.01 0.027
Thiamine-HCl x 2 H2O 10.0 mg 0.01 0.027
Nicotinamide 10.0 mg 0.01 0.082
D-Calcium pantothenate 10.0 mg 0.01 -
Vitamin B12 0.2 mg 2.0e-4 1.5e-4
p-Aminobenzoic acid 10.0 mg 0.01 0.073
Distilled water 1000.0 ml - -
1 Store in the dark and cold (5°C).

Metadata

Last modified: 23.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2 - 7.4
Edit in Medium builder

Associated strains

All strains for this medium: 16
Strains with modifications: 16

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 22.655 387.668
Agar 15 44.599
MgCl2 x 6 H2O 4.807 23.643
Na2SO4 3.78 26.612
CaCl2 1.063 9.582
Show more
CSV
JSON

Cultivation metadata from 16 strains BacDive

pH 8 - 10 2 strains
pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 4 16 strains
oxygen aerobe 5 strains
Show more