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DSMZ

DSMZ 611 . PYROBACULUM (BS) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 611 #

ml
Equipment needed: Autoclave Gassing station Serum bottle
Compound Amount Unit Conc. [g/L] Conc. [mM]
Synthetic seawater mix 125.00 ml - -
Modified Wolin's mineral solution 10.00 ml - -
Fe(NH4)2(SO4)2 x 6 H2O
(0.01% w/v) 		2.00 ml 2.0e-4 -
NH4Cl 0.25 g 0.25 4.674
KH2PO4 0.07 g 0.07 0.514
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
KNO3 1.00 g 1 9.891
Yeast extract 0.50 g 0.5 -
NaHCO3 2.00 g 2 23.808
Distilled water 875.00 ml - -
1 Dissolve ingredients (except nitrate, yeast extract and bicarbonate) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Adjust pH to 7.0 and dispense under 100% N2 gas atmosphere into serum vials (e.g., 20 ml medium per 100 ml bottle). Replace gas phase with 80% H2 and 20% CO2 gas mixture and autoclave. Prior to inoculation add nitrate and yeast extract from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.0 - 7.2.

Synthetic seawater mix #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 47.15 g 47.15 806.81
MgCl2 x 6 H2O 18.10 g 18.1 89.03
MgSO4 x 7 H2O 7.00 g 7 28.4
CaCl2 x 2 H2O 3.13 g 3.13 21.29
Na2SO4 3.24 g 3.24 22.811
KCl 1.20 g 1.2 16.097
Na2CO3 0.10 g 0.1 0.944
NaBr 0.10 g 0.1 0.972
KBr 80.00 mg 0.08 0.672
SrCl2 x 6 H2O 72.00 mg 0.072 0.27
H3BO3 52.00 mg 0.052 0.841
Na2HPO4 8.10 mg 0.008 0.057
NaF 2.40 mg 0.002 0.057
Na-silicate 0.40 mg 4.0e-4 0.003
KI 50.00 µg 5.0e-5 1.9e-4
Distilled water 1000.00 ml - -

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 05.04.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Gassing station, Serum bottle
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.263 11.129
NaHCO3 2 23.808
KNO3 1 9.891
Yeast extract 0.5 -
Na2SO4 0.405 2.851
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain