Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
This strain uses the standard medium without modifications.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution A | 843 | ml | - | - | |
Solution B | 50 | ml | - | - | |
Solution C | 50 | ml | - | - | |
Solution D | 40 | ml | - | - | |
Solution E | 10 | ml | - | - | |
Solution F | 10 | ml | - | - | |
1 Sparge solution A with 100% CO2 gas for 30 - 45 min to make it anoxic, then distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Autoclave separately solutions B, C, E and F under 100% N2 gas, and solution D under 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Adjust pH of the complete medium to 6.9, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
MgSO4 x 7 H2O | 0.10 | g | 0.119 | 0.481 |
(NH4)2SO4 | 0.50 | g | 0.593 | 4.489 |
Fe(NH4)2(SO4)2 | 0.04 | g | 0.047 | - |
Na2MoO4 x 2 H2O
(0.1% w/v) |
2.40 | ml | 0.003 | 0.012 |
Na2SeO3 x 5 H2O
(0.1% w/v) |
0.15 | ml | 1.8e-4 | 6.8e-4 |
Tryptone | 5.00 | g | 5.931 | - |
Yeast extract | 5.00 | g | 5.931 | - |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.9e-4 | 0.002 |
Distilled water | 840.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
K2HPO4 | 7.0 | g | 140 | 803.785 |
KH2PO4 | 4.5 | g | 90 | 661.351 |
Distilled water | 50.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Glucose | 18 | g | 360 | 1998.22 |
Distilled water | 50 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na2CO3 | 2 | g | 50 | 471.752 |
Distilled water | 40 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
L-Cysteine HCl x H2O | 0.3 | g | 30 | 170.808 |
Distilled water | 10.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na2S x 9 H2O | 0.3 | g | 30 | 124.905 |
Distilled water | 10.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 6.9 |
Equipment needed: | Autoclave, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 4 |
No modifications necessary for this strain.
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Glucose | 17.946 | 99.612 |
K2HPO4 | 6.979 | 40.069 |
Yeast extract | 4.985 | - |
Tryptone | 4.985 | - |
KH2PO4 | 4.487 | 32.969 |
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