603
603 .
ANCALOMICROBIUM MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Stella humosa DSM 5900
* Modification: Add 0.1 g/l yeast extract.
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 28 °C
Main sol. 603 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 0.250 | g | 0.248 | 1.873 | |
| Glucose | 0.250 | g | 0.248 | 1.374 | |
| Vitamin solution | 10.000 | ml | - | - | |
| Hutner's salts | 20.000 | ml | - | - | |
| Na2HPO4 | 0.071 | g | 0.07 | 0.495 | |
| Distilled water | 980.000 | ml | - | - | |
| Agar , for solid medium | 15.000 | g/l | 15 | 44.599 | |
| Yeast extract* | 0.100 | g/l | - | - | |
| 1 pH 7 | |||||
| 2 Make up the medium without the vitamin solution and autoclave it. The filter-sterilized vitamin solution is added to the cooled medium. The medium may be solidified by adding 15 g/l agar. | |||||
Solution occurs in 2
other media (first defined in Medium 603).
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 | |
| Biotin | 2.0 | mg | 0.002 | 0.008 | |
| Thiamine-HCl x 2 H2O | 5.0 | mg | 0.005 | 0.013 | |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 | |
| Folic acid | 2.0 | mg | 0.002 | 0.005 | |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 | |
| Nicotinamide | 5.0 | mg | 0.005 | 0.041 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Filter-sterilized | |||||
Solution occurs in 2
other media (first defined in Medium 603).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
10.000 | g | 10 | 52.318 | |
| MgSO4 x 7 H2O | 29.700 | g | 29.7 | 120.499 | |
| CaCl2 x 2 H2O | 3.335 | g | 3.335 | 22.685 | |
| (NH4)6Mo7O24 x 4 H2O | 9.250 | mg | 0.009 | - | |
| FeSO4 x 7 H2O | 99.000 | mg | 0.099 | 0.356 | |
| "Metals 44" | 50.000 | ml | - | - | |
| Distilled water | 950.000 | ml | - | - | |
| 1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4. | |||||
Solution occurs in 34
other media (first defined in Medium 590).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na-EDTA | 250.0 | mg | 0.25 | 0.744 | |
| ZnSO4 x 7 H2O | 1095.0 | mg | 1.095 | 3.808 | |
| FeSO4 x 7 H2O | 500.0 | mg | 0.5 | 1.798 | |
| MnSO4 x H2O | 154.0 | mg | 0.154 | 0.911 | |
| CuSO4 x 5 H2O | 39.2 | mg | 0.039 | 0.157 | |
| Co(NO3)2 x 6 H2O | 24.8 | mg | 0.025 | 0.085 | |
| Na2B4O7 x 10 H2O | 17.7 | mg | 0.018 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. | |||||
Metadata
| Last modified: | 23.03.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| MgSO4 x 7 H2O | 0.588 | 2.386 |
| (NH4)2SO4 | 0.248 | 1.873 |
| Glucose | 0.248 | 1.374 |
| Nitrilotriacetic acid | 0.198 | 1.036 |
| Show more | ||
