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DSMZ

DSMZ 602 . HALOMONAS SUBGLACIESCOLA

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 602 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 80.0 g 80 1368.93
MgSO4 x 7 H2O 9.5 g 9.5 38.543
KCl 0.5 g 0.5 6.707
CaCl2 x 2 H2O 0.2 g 0.2 1.36
(NH4)2SO4 0.1 g 0.1 0.757
KNO3 0.1 g 0.1 0.989
Yeast extract 1.0 g 1 -
Distilled water 960.0 ml - -
Hutner's salts 20.0 ml - -
Vitamin solution 1.0 ml - -
Phosphate supplement 20.0 ml - -
1 pH 8.0
2 Autoclave and add, on cooling 20.0 ml sterile Hutner's salts (see medium 590), 1.0 ml vitamin solution, and 20.0 ml sterile phosphate supplement.

Vitamin solution #

1000
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 10 mg 0.01 0.007
Biotin 2 mg 0.002 0.008
Thiamine-HCl x 2 H2O 10 mg 0.01 0.027
Calcium pantothenate 5 mg 0.005 0.01
Folic acid 2 mg 0.002 0.005
Nicotinamide 5 mg 0.005 0.041
Pyridoxine hydrochloride 10 mg 0.01 0.049
Distilled water 1000 ml - -
1 Filter-sterilized.

Phosphate supplement #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 50 mg 2.5 14.353
KH2PO4 50 mg 2.5 18.371
Distilled water 20 ml - -

Hutner's salts #

1000
Solution occurs in 24 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 0.007
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
"Metals 44" 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid.
2 Adjust pH to 7.0 with KOH (~7.3 g).
3 Dissolve other salts separately and combine.
4 Adjust pH to 6.8 with NaOH or H2SO4.

"Metals 44" #

1000
Solution occurs in 34 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 80 1368.93
Yeast extract 1 -
MgSO4 x 7 H2O 0.594 2.41
KCl 0.5 6.707
Nitrilotriacetic acid 0.2 1.046
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains
temperature psychrophilic 1 strain