58
58 .
BIFIDOBACTERIUM MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Bifidobacterium sp. DSM 108971
* Modification: Plus haemine (50µg/ml) plus Vit K1 plus Na-lactate (0.5%)
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 1-2 days
anaerobic
37 °C
pH 7.00
Main sol. 58 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Casein peptone
(tryptic digest) |
10.00 | g | 10 | - | |
| Yeast extract | 5.00 | g | 5 | - | |
| Meat extract | 5.00 | g | 5 | - | |
| Bacto Soytone | 5.00 | g | 5 | - | |
| Glucose | 10.00 | g | 10 | 55.506 | |
| K2HPO4 | 2.00 | g | 2 | 11.483 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| MnSO4 x H2O | 0.05 | g | 0.05 | 0.296 | |
| Tween 80 | 1.00 | ml | - | - | |
| NaCl | 5.00 | g | 5 | 85.558 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | - | |
| Salt solution | 40.00 | ml | - | - | |
|
Resazurin
(25 mg/100ml) |
4.00 | ml | - | - | |
| Haemin* | 50.00 | mg/l | - | - | |
| Na-lactate* | 5.00 | g/l | - | - | |
| Distilled water | 950.00 | ml | - | - | |
| 1 The cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 6.8 using 8 N NaOH. Distribute under N2 and autoclave. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 |
| K2HPO4 | 1.00 | g | 1 | 5.741 |
| KH2PO4 | 1.00 | g | 1 | 7.348 |
| NaHCO3 | 10.00 | g | 10 | 119.038 |
| NaCl | 2.00 | g | 2 | 34.223 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 10 | 55.506 |
| Casein peptone | 10 | - |
| Bacto Soytone | 5 | - |
| Meat extract | 5 | - |
| Yeast extract | 5 | - |
| Show more | ||
Cultivation metadata from 222 strains
| pH | 6 - 8 | 20 strains |
| pH | 4 - 6 | 14 strains |
| pH | 8 - 10 | 3 strains |
| pH | 2 - 4 | 2 strains |
| pH | 10 - 12 | 1 strain |
| Show more | ||