Name: BIFIDOBACTERIUM MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 58 #

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Casein peptone (tryptic digest)	10.00	g	10	-
Yeast extract	5.00	g	5	-
Meat extract	5.00	g	5	-
Bacto Soytone	5.00	g	5	-
Glucose	10.00	g	10	55.506
K₂HPO₄	2.00	g	2	11.483
MgSO₄ x 7 H₂O	0.20	g	0.2	0.811
MnSO₄ x H₂O	0.05	g	0.05	0.296
Tween 80	1.00	ml	-	-
NaCl	5.00	g	5	85.558
L-Cysteine HCl x H₂O	0.50	g	0.5	-
Salt solution	40.00	ml	-	-
Resazurin (25 mg/100ml)	4.00	ml	-	-
Distilled water	950.00	ml	-	-
1 The cysteine are added after the medium has been boiled and cooled under CO₂. Adjust pH to 6.8 using 8 N NaOH. Distribute under N₂ and autoclave.

Salt solution #

1000

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
CaCl₂ x 2 H₂O	0.25	g	0.25	1.701
MgSO₄ x 7 H₂O	0.50	g	0.5	2.029
K₂HPO₄	1.00	g	1	5.741
KH₂PO₄	1.00	g	1	7.348
NaHCO₃	10.00	g	10	119.038
NaCl	2.00	g	2	34.223
Distilled water	1000.00	ml	-	-

All strains for this medium:	211
Strains with modifications:	20

CSV

JSON