54b .
CLOSTRIDIUM SP. MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Glucose | 20 | g | ||
|
|
Yeast extract | 10 | g | ||
|
|
CaCO3
(light precipitate) |
20 | g | ||
|
|
Agar
, for solid medium
optional |
17 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Dissolve ingredients (note that most of the carbonate remains suspended) and sparge medium with 100% N2 gas for 30 -45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials vials while stirring to keep carbonate suspended and autoclave. | |||||