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DSMZ

DSMZ 541 . SULFUROSPIRILLUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Sulfurospirillum cavolei DSM 18149

* Modification: Supplement medium after autoclaving with 1.00 ml/l Wolin's vitamin solution 10x (see medium 120) sterilized by filtration. Prior to inoculation reduce the complete medium with 10 - 20 mg/l sodium dithionite (added from a 5% w/v stock solution freshly prepared under 100% N2 gas and filter-sterilized) instead of using cysteine.

Growth conditions: anaerobic 30 °C

Main sol. 541 #

ml
Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 952.0 ml - -
Solution B 30.0 ml - -
Solution C 20.0 ml - -
Solution D* 2.0 ml - -
Wolin's vitamin solution (10x)* 1.0 ml - -
Na-dithionite solution (5% w/v)* 0.5 ml - -
1 Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C should be prepared under 100% N2 gas and sterilized by filtration. Solution D is autoclaved under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B to D are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.2, if necessary.

Solution A #

952
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.36 g 1.429 10.498
MgSO4 x 7 H2O 0.37 g 0.389 1.577
CaCl2 x 2 H2O 0.10 g 0.105 0.714
NH4Cl 0.27 g 0.284 5.302
Trace element solution SL-10 2.00 ml - -
Distilled water 950.00 ml - -

Solution B #

30
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1.5 g 50 471.752
Distilled water 30.0 ml - -

Solution C #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-fumarate 4 g 200 1249.72
Distilled water 20 ml - -

Solution D #

2
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine HCl x H2O 0.07 g 35 199.276
Distilled water 2.00 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Wolin's vitamin solution (10x) * #

1000
Solution occurs in 308 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Na-dithionite solution (5% w/v) * #

10
Solution occurs in 7 other media (first defined in Medium 829).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH
(1 N) 		0.5 ml - -
Na2S2O4 0.5 g 50 287.175
Distilled water 9.5 ml - -
1 Add 1 N NaOH to distilled water and make the solution anoxic by sparging with 100% N2 gas. Then dissolve the Na-dithionite and filter-sterilize the solution into anoxic Hungate tubes. The final pH of the solution should be between 9 and 10. Store the prepared solution in a dark and cool place. Prepare only small amounts of stock solution, as Na-dithionite decomposes rapidly.

Metadata

Last modified: 19.08.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 4
Strains with modifications: 2
Sulfurospirillum cavolei DSM 18149

-  Solution D
+  1 ml Wolin's vitamin solution (10x)
+  0.5 ml Na-dithionite solution (5% w/v)

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Na2-fumarate 3.984 24.895
Na2CO3 1.494 14.096
KH2PO4 1.355 9.954
MgSO4 x 7 H2O 0.369 1.495
NH4Cl 0.269 5.028
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Cultivation metadata from 4 strains BacDive

pH 6 - 8 1 strain
phyla 1 4 strains
oxygen anaerobe 3 strains
oxygen facultative anaerobe 1 strain
oxygen microaerophile 1 strain
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