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DSMZ

DSMZ 514f . MARINE BROTH (ROTH CP73)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Binariimonas pacifica DSM 28646

This strain uses the standard medium without modifications.

Growth conditions: 3-7 days aerobic 28 °C

Main sol. 514f #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5.00 g 5 -
Yeast extract 1.00 g 1 -
Fe(III) citrate 0.10 g 0.1 0.408
NaCl 19.40 g 19.4 331.964
MgCl2 8.80 g 8.8 92.427
Na2SO4 3.24 g 3.24 22.811
CaCl2 1.80 g 1.8 16.219
KCl 0.55 g 0.55 7.378
NaHCO3 0.16 g 0.16 1.905
KBr 0.08 g 0.08 0.672
SrCl2 34.00 mg 0.034 0.214
H3BO3 22.00 mg 0.022 0.356
Na-silicate 4.00 mg 0.004 0.033
NaF 2.40 mg 0.002 0.057
(NH4)NO3 1.60 mg 0.002 0.02
Na2HPO4 8.00 mg 0.008 0.056
Distilled water 1000.00 ml - -
Agar , if required 15.00 g/l 15 44.599
1 Final pH should be 7.6 ± 0.2 at 25°C. Add 15.0 g/l agar for solid media. We recommend using the medium supplied by Carl Roth GmbH + Co. KG for Marinomonas mediterranea DSM 23531.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4 - 7.8
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Associated strains

All strains for this medium: 83
Strains with modifications: 2
Binariimonas pacifica DSM 28646

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.4 331.964
Agar 15 44.599
MgCl2 8.8 92.427
Peptone 5 -
Na2SO4 3.24 22.811
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Cultivation metadata from 83 strains BacDive

pH 6 - 8 10 strains
pH 4 - 6 9 strains
pH 8 - 10 3 strains
pH 10 - 12 1 strain
phyla 5 88 strains
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