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DSMZ

DSMZ 507a . CARBOXYDOTHERMUS SIDEROPHILUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Carboxydothermus siderophilus DSM 21278

This strain uses the standard medium without modifications.

Growth conditions: anaerobic 65 °C

Main sol. 507a #

ml
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.33 g 0.326 4.378
MgCl2 x 6 H2O 0.52 g 0.514 2.53
CaCl2 x 2 H2O 0.29 g 0.287 1.951
NH4Cl 0.33 g 0.326 6.102
KH2PO4 0.33 g 0.326 2.399
Na2-9,10-anthraquinone-2,6-disulfonate
(TCI A0308) 		8.25 g 8.16 -
Trace element solution SL-4 10.00 ml - -
NaHCO3 1.00 g 0.989 11.774
Yeast extract 0.20 g 0.198 -
Wolin's vitamin solution (10x) 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients except bicarbonate, yeast extract, vitamins and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add yeast extract, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. After completing the medium adjust pH to 6.8 - 7.0.
2 Inoculated vessels are pressurized with sterile carbon monoxide gas to 1 bar overpressure.

Trace element solution SL-4 #

1000
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 307 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 27.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.9
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 50 % CO
  • 50 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Carboxydothermus siderophilus DSM 21278

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2-9,10-anthraquinone-2,6-disulfonate 8.16 -
NaHCO3 0.989 11.774
MgCl2 x 6 H2O 0.514 2.53
NH4Cl 0.326 6.102
KCl 0.326 4.378
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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